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Image Search Results
Journal: Cancer Immunology, Immunotherapy : CII
Article Title: Immune activation of the monocyte-derived dendritic cells using patients own circulating tumor cells
doi: 10.1007/s00262-022-03189-2
Figure Lengend Snippet: The study outline summarizes the monocyte-derived dendritic cells (Mo-DCs) production in CliniMACS Prodigy® machine in a closed tubes system under sterile conditions. CliniMACS Prodigy® technology is used for automatic immunomagnetic antibody-based separation of CD14 + cells out of the leukapheresis (up to 100 mL). CD14 + cells are further cultured in vitro under the “controlled” in tube/bag conditions to generate immature Mo-DCs (iMo-DCs) and mature Mo-DCs (mMO-DCs). Protocol for mMo-DCs production included several modifications in comparison to the standard CliniMACS Prodigy® procedures. The changes were considering different cultivation length, different “activation strategies” using circulating tumor cell lysate (CTC lysate) to produce mMo-DCs
Article Snippet: Monocytes from the remaining PBMC were separated using
Techniques: Derivative Assay, Sterility, Cell Culture, In Vitro, Comparison, Activation Assay